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Pixel Club: Morphology-Guided Graph Search for Untangling Objects in Microscopy Images
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Tammy Riklin Raviv (EE, Ben Gurion University)
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Tuesday, 2.6.2015, 11:30
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EE Meyer Building 1061
In the talk I will present a novel framework for high-throughput cell lineage analysis in time-lapse microscopy images. The proposed algorithm ties together two fundamental aspects of cell lineage construction, namely cell segmentation and tracking, via a Bayesian inference of dynamic models. The proposed contribution exploits the Kalman inference problem by estimating the time-wise cell shape uncertainty in addition to cell trajectory. These inferred cell properties are combined with the observed image measurements within a fast marching (FM) algorithm, to achieve posterior probabilities for cell segmentation and association. An original idea for cell mitosis detection, which is not specific to a particular dataset and does not require training will also presented. Highly accurate results, with respect to the state-of-the-art, on different cell-tracking datasets were obtained.
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